rabbit anti bovine  (Bio-Rad)

Journal: International Journal of Molecular Sciences

Article Title: Roburic Acid as a Therapeutic Candidate: Antiproliferative Activity and Secondary Cell Death Response in Colorectal Cancer Cells

doi: 10.3390/ijms27052478

Figure Lengend Snippet: Effect of roburic acid (RA) on Ki-67 expression in colorectal cancer (CRC) cell lines. Ki-67 immunofluorescence staining in DLD-1, HCT-116, and HT-29 following 48 h of exposure to RA at concentrations corresponding to 0.5 × IC 50 and IC 50 . Quantitative data are presented as medians with interquartile ranges, minimums, and maximums. Statistical analysis was performed using the Kruskal–Wallis test, followed by Dunn’s post hoc test; an asterisk indicates significant differences relative to untreated controls (*) ( p < 0.05).

Article Snippet: Samples were then incubated for 1 h with a rabbit anti-Ki-67 primary antibody (Thermo Fisher, MA5-14520; 1:250 dilution in PBS supplemented with 1% bovine serum albumin and 0.5% Tween-20), followed by a 1 h incubation with an Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody (Thermo Fisher, A-11008; 1:500 dilution).

Techniques: Expressing, Immunofluorescence, Staining